Research Article

A Synthetic Strategy for Conjugation of Paromomycin to Cell-Penetrating Tat(48-60) for Delivery and Visualization into Leishmania Parasites

Figure 3

Fluorescence of Leishmania donovani promastigotes incubated with Bodi Fluor 488-labeled PMM-Tat conjugate (9 μM, 4 h, 26°C) and stained with DAPI (5 μg/mL) immediately before observation, unfixed. Settings: conjugate (green, = 488 nm/ = 520 nm); DAPI (blue, = 350 nm/ = 460 nm). Bar = 20 μm. Experiment is representative of two other ones performed independently.