Recent Advances in Cotton Genomics

Table 3

Upland cotton BAC and BIBAC libraries that have been published or are accessible to the public (as of May 2007).

Genotype	Mean insert size (kb)	No. of clones	Genome equivalents	Vector(a)	Cloning site	References/locations where libraries are available
Tamcot HQ95	93	51,353	2.3x	pBeloBAC11	HindIII	http://hbz7.tamu.edu
Auburn 623	140	44,160	2.7x	pBeloBAC11	BamHI	http://hbz7.tamu.edu
Texas Marker-1	130	76,800	4.4x	pCLD04541	BamHI	http://hbz7.tamu.edu
Texas Marker-1	175	76,800	6.0x	pECBAC1	EcoRI	http://hbz7.tamu.edu
Maxxa	137	129,024	8.3x	pCUGI-1	HindIII	[79]
0-613-2R	130	97,825	5.7x	pIndigoBAC-5	HindIII	[80]

(a) The vectors, pBeloBAC11 (Kim et al. [81]), pECBAC1 (Frijters et al. [82]), pCUGI-1 [79], and pIndigoBAC-5 (http://www.epibio.com/item.asp?ID=328), are BAC vectors whereas pCLD04541 is plant-transformation-competent BIBAC vector (http://www.jic.bbsrc.ac.uk/staff/ian-bancroft/vectorspage.htm; [83]) that can be directly transformed into cotton plants via Agrobacterium.