Soybean Genomics: Developments through the Use of Cultivar “Forrest”

Table 7

Some of the BACs, mutant and nonmutant soybean lines to be transformed for complementation.

BIBAC clone names	Phenotypes	Insert size kbp	Dominant?
Gm-SIU1-B100B10	Rhg4 bigenic resistance to SCN^(a)	140	Yes
Gm-SIU1-B73P06	rhg1 bigenic resistance to SCN and Rfs2 for SDS^(a)	79	Co-
Gm-SIU2-H050N07	Rpg1-b resistance to bacterial pustule^(b)	110	Yes
Gm-SIU1-B54E07	T tawny pubescence; flavonoid-3-monoxygenase^(c)	82	Yes
Gm-SIU2-H04P03	W1 White flower and black hila color^(d)	153	No
Gm-SIU2-H82CO8	Rfs1 root resistance to SDS	130	Yes
Gm-SIU1-TBD	Rps4 resistance to Phytophthora root rot	120	Yes

^(a)Rhg4 and rhg1 each encodes transmembrane receptor-like kinase. Resistant and susceptible alleles differ by 3–6 amino acid changes and 23 base changes. There are mutant lines derived from Forrest.
^(b)Rpg1-b encodes a nucleotide binding leucine rich repeat protein.
^(c)T encodes flanonoid-3 monoxygenase (EC1.13.14.21). The recessive genes differ from the dominant by deletion of a single C nucleotide. There are mutant lines.
^(d)W encodes an unknown enzyme, probably a glycosidase.