A Solanum lycopersicum Solanum pimpinellifolium Linkage Map of Tomato Displaying Genomic Locations of R-Genes, RGAs, and Candidate Resistance/Defense-Response ESTs
Table 1
Listof ESTs mapped in the Solanum lycopersicum × S. pimpinellifolium F2 population, their putative function,
chromosomal location, and copy
number.
EST clone
utative function
Chr.
opy no.
cTOF3A14
C146883
Cytosolic Cu, Zn Superoxide dismutase, S. lycopersicum
1
2
cTOE7J7a
C139397
Endo-1,4-beta-glucanase, S. lycopersicum
1
6
cLED27E12
C19568
Cold
acclimation protein WCOR413-like protein form, O. sativa
1
2
cTOE6F10
C139034
Lipoxygenase, S. lycopersicum
1
5
cLEG9N2
C45935
Subunit A
of ferredoxin-thioredoxin reductase, S.
tuberosum
1
1
cLES9N20
C79709
ASC1 (Alternaria stem canker resistance protein), S. lycopersicum
1
1
cLEC6O2
C11013
Polyamine
oxidase, A. thaliana
1
1
cTOF20P4
C142906
Carotenoid
cleavage dioxygenase 1-2, S.
lycopersicum
1
5
cLEZ11K12
C98684
Snakin2
precursor, S. lycopersicum
1
1
cTOA9E13
C117653
Squalene synthase, C.
annuum
1
5
cTOA9C11
C117644
Similar to
WRKY transcription factor Nt-SubD48, N.
tabacum
2
1
cLET10E15
C79822
Acidic 26kDa endochitinase precursor, S. lycopersicum
2
1
cTOF19J9
C142319
Phosphoribosylanthranilate
isomerase, A. thaliana
2
1
cLEY1K9
C97179
Pathogen-inducible
alpha-dioxygenase, N. attenuata
2
4
cLEW11E20
C89000
Resistance complex protein I2C-3, S. lycopersicum
2
7
cTOF16A9
C141311
Calmodulin 3 protein, S. lycopersicum
3
9
cLER17H16
C71298
Elicitor-inducible cytochrome P450, N. tabacum
3
1
cTOF18P1
C142154
Serine palmitoyltransferase, S. tuberosum
3
3
cLEX12O16
C92852
Ethylene response factor 5, S. lycopersicum
3
6
cTOE2F15
C137984
Catalase isozyme 1, S. lycopersicum
3
1
cTOF29J22
C145412
4-coumarate-coA ligase 1, S. tuberosum
3
2
cLEX10F20
C92172
Ethylene response factor 1, S. lycopersicum
3
4
cTOF14B17
C141010
Anthocyanin 5-O-glucosyltransferase, S. sogarandinum
4
1
cLED15E5
C16128
Shikimate kinase chloroplast precursor, S. lycopersicum
4
1
cLEN13D5
C66215
Chorismate synthase 1 precursor, S. lycopersicum
4
4
cTOS21D12
C163577
Similar to
heat shock factor, N. tabacum
4
3
cTOF10N11
C140057
Myo-inositol-1-phosphate synthase, S. lycopersicum
9-cis-epoxycarotenoid dioxygenase, S. lycopersicum
11
7
cLED23K21
C18512
Resistance complex protein I2C-5, S. lycopersicum
11
>10
cLES18N16
C76694
Phosphatidylinositol 4-kinase, S. tuberosum
11
2
cTOS21D14
C163579
WRKY
transcription factor IId-2, S. lycopersicum
12
1
cLPT1G11
C109877
S-adenosyl-l-homocysteine
hydrolase, S. lycopersicum
12
4
cLEZ15E8
C98979
Extensin class I, S. Lycopersicum
12
>10
cLEW25D9
C90989
Glutamine synthetase, S. lycopersicum
12
3
olanaceae Genome Network (SGN) can be accessed at http://www.sgn.cornell.edu/. he putative function of each EST has been derived
from Computational Biology and Functional Genomics Laboratory web site (http://compbio.dfci.harvard.edu/tgi/cgi-bin/tgi/est_report.pl), used to be maintained at The Institute for Genomic
Research (TIGR). (Computational Biology and
Functional Genomics Laboratory). he exact or approximate copy number of ESTs in tomato
genome was determined based on the number of hybridized bands on Southern blot
gels and may be varied in different labs. Where there is a “/” sign, the
figures in the left side denote the number of copies in S. lycopersicum parent and those in the right side denote the
number of copies in S. pimpinellifolium parent.