Reproducible RNA Preparation from Sugarcane and Citrus for Functional Genomic Applications

Figure 1

Assessment of the integrity of total RNA isolated by the TENS-PCI method, and the two kits, FastRNA Pro Green and RNeasy Plant Mini, from sugarcane leaf, stem, and root tissues, using chip-based electrophoretic separation with a bioanalyzer. A total of 100 ng of RNA was loaded per well. Representative RNA profiles corresponding to the three tissues are shown. Gel-like images of RNA from each tissue, generated from the RNA profiles, were included for clarity on the right side. The Agilent RNA 6000 Nano ladder is included for sizing. SS and LS represent the small and large ribosomal subunits, respectively. Numeric values represent mean and standard error of two biological replicates and at least three technical repeats.