Reproducible RNA Preparation from Sugarcane and Citrus for Functional Genomic Applications
Figure 3
Northern analysis of the expression levels of sugarcane (a) and sweet orange (b) lines overexpressing a mammalian gene. Total RNA was isolated from leaf tissues by the TENS-PCI, the RNeasy guanidine-based (GB), and phenol-based (PB) methods. GB-ITC refers to guanidine isothiocyanate and GB-C to guanidine hydrochloride. RNA (10 g each lane) was fractionated, blotted, hybridized, washed, and imaged as described in Material and Methods.
