Methodology Report
Reproducible RNA Preparation from Sugarcane and Citrus for Functional Genomic Applications
Table 2
Comparison of the TENS-PCI method with other RNA isolation procedures used for plant species with high levels of secondary metabolites.
| | Method | RNA yield (g/mg tissue) | RNA purity | | | | | Leaf | | Leaf | Stem | Root | A260 : A230 | A260 : A280 |
| | Sugarcane (Saccharum spp. hybrid) (present study) | | | | | | | TENS-PCI: Micro-scale | 0.59 ± 0.07 | 0.44 ± 0.05 | 0.45 ± 0.03 | 1.99 ± 0.07 | 1.97 ± 0.09 | | Macro-scale | 2.42 ± 0.15 | 3.92 ± 0.27 | 0.99 ± 0.08 | 2.01 ± 0.07 | 1.98 ± 0.06 | | RNeasy Plant Mini Kit | | | | | | | Guanidine isothiocyanate | 0.10 ± 0.04 | NA | NA | 2.30 ± 0.08 | 1.95 ± 0.10 | | Guanidine hydrochloride | 0.11 ± 0.04 | NA | NA | 1.20 ± 0.06 | 1.92 ± 0.11 | | FastRNA Pro Green Kit (Phenol-based) | 0.11 ± 0.03 | NA | NA | 1.17 ± 0.05 | 1.79 ± 0.07 | | Sweet orange (Citrus sinensis Pers.) (present study) | | | | | | | TENS-PCI: Macro-scale | 2.29 ± 0.11 | NA | NA | 2.13 ± 0.01 | 2.07 ± 0.01 | | Woody plants | | | | | | | (1) Grapevine (Vitis spp.) [44] | | | | | | | Rapid CTAB | 0.11 ± 0.05 | 0.11 ± 0.03 | NA | 2.29 ± 0.12 | 1.89 ± 0.03 | | RNeasy kit | 0.04 ± 0.01 | 0.04 ± 0.01 | NA | 0.36 ± 0.05 | 1.07 ± 0.04 | | Guanidine | 0.12 ± 0.02 | 0.10 ± 0.02 | NA | 1.11 ± 0.08 | 1.77 ± 0.10 | | (2) Lemon tree (Citrus limon Burm.f.) | 0.14 ± 0.03 | 0.06 ± 0.01 | NA | 2.28 ± 0.14 | 2.01 ± 0.03 | | Rapid CTAB [44] | | | | | | | (3) Plum (Prunus domestica L.) | 0.18 ± 0.01 | 0.21 ± 0.04 | NA | 2.23 ± 0.11 | 2.03 ± 0.02 | | Rapid CTAB [44] | | | | | | | (4) London plane tree (Platanus acerifolia Willd.) [6] | | | | | | | Guanidine | 0.20 ± 0.004 | NA | NA | 1.49 ± 0.07 | 1.35 ± 0.11 | | SDS/phenol | 0.12 ± 0.004 | NA | NA | 1.47 ± 0.06 | 1.49 ± 0.89 | | CTAB | 0.26 ± 0.007 | NA | NA | 1.61 ± 0.07 | 1.62 ± 0.79 |
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Data represent mean and standard error of at least 6 biological replicates and 6 technical repeats.
NA: data not available.
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