Heterologous Expression of a Membrane-Spanning Auxin Importer: Implications for Functional Analyses of Auxin Transporters

<table>Blue native PAGE analysis of AUX1 expression in insect cells. N-HA-AUX1 membrane fractions from insect (Sf9) cells were solubilised in 0.1% (w/v) DDM in the presence of increasing concentrations of NaCl and were resolved by BN-PAGE on 6–16% gradient gels, transferred to PVDF and identified by immunoblotting with anti-HA antibodies. Molecular weight (kDa) of marker proteins is denoted at the left-hand side of the figure.</table>

International Journal of Plant Genomics

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Figure 4

Figure 4: Heterologous Expression of a Membrane-Spanning Auxin Importer: Implications for Functional Analyses of Auxin Transporters 