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International Journal of Plant Genomics
Volume 2009 (2009), Article ID 848145, 8 pages
Research Article

Heterologous Expression of a Membrane-Spanning Auxin Importer: Implications for Functional Analyses of Auxin Transporters

1School of Biomedical Sciences, University of Nottingham, Queen's Medical Centre, Nottingham NG7 2UH, UK
2School of Biosciences, University of Nottingham, Sutton Bonington Campus, Leics LE12 5RD, UK

Received 22 January 2009; Revised 9 March 2009; Accepted 27 April 2009

Academic Editor: Hikmet Budak

Copyright © 2009 David John Carrier et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Linked References

  1. M. J. Bennett, A. Marchant, H. G. Green et al., “Arabidopsis AUX1 gene: a permease-like regulator of root gravitropism,” Science, vol. 273, no. 5277, pp. 948–950, 1996. View at Google Scholar
  2. E. P. Maher and S. J. B. Martindale, “Mutants of Arabidopsis thaliana with altered responses to auxins and gravity,” Biochemical Genetics, vol. 18, no. 11-12, pp. 1041–1053, 1980. View at Google Scholar
  3. R. Swarup, J. Kargul, A. Marchant et al., “Structure-function analysis of the presumptive Arabidopsis auxin permease AUX1,” The Plant Cell, vol. 16, no. 11, pp. 3069–3083, 2004. View at Publisher · View at Google Scholar
  4. H. M. Saier Jr., “Families of transmembrane transporters selective for amino acids and their derivatives,” Microbiology, vol. 146, part 8, pp. 1775–1795, 2000. View at Google Scholar
  5. I. D. Kerr and M. J. Bennett, “New insight into the biochemical mechanisms regulating auxin transport in plants,” The Biochemical Journal, vol. 401, no. 3, pp. 613–622, 2007. View at Publisher · View at Google Scholar
  6. P. G. G. A. de Ruyter, O. P. Kuipers, and W. M. de Vos, “Controlled gene expression systems for Lactococcus lactis with the food-grade inducer nisin,” Applied and Environmental Microbiology, vol. 62, no. 10, pp. 3662–3667, 1996. View at Google Scholar
  7. F. L. Graham, J. Smiley, W. C. Russell, and R. Nairn, “Characteristics of a human cell line transformed by DNA from human adenovirus type 5,” Journal of General Virology, vol. 36, no. 1, pp. 59–72, 1977. View at Google Scholar
  8. J. Ponten and E. Saksela, “Two established in vitro cell lines from human mesenchymal tumours,” International Journal of Cancer, vol. 2, no. 5, pp. 434–447, 1967. View at Google Scholar
  9. O. Boussif, F. Lezoualc'h, M. A. Zanta et al., “A versatile vector for gene and oligonucleotide transfer into cells in culture and in vivo: polyethylenimine,” Proceedings of the National Academy of Sciences of the United States of America, vol. 92, no. 16, pp. 7297–7301, 1995. View at Publisher · View at Google Scholar
  10. L. A. King and R. D. Possee, The Baculovirus Expression System: A Laboratory Guide, Chapman & Hall, London, UK, 1992.
  11. H. Schagger, W. A. Cramer, and G. Vonjagow, “Analysis of molecular masses and oligomeric states of protein complexes by blue native electrophoresis and isolation of membrane protein complexes by two-dimensional native electrophoresis,” Analytical Biochemistry, vol. 217, no. 2, pp. 220–230, 1994. View at Publisher · View at Google Scholar
  12. D. J. Carrier, N. T. A. Bakar, R. Swarup et al., “The binding of auxin to the arabidopsis auxin influx transporter AUX1,” Plant Physiology, vol. 148, no. 1, pp. 529–535, 2008. View at Publisher · View at Google Scholar
  13. J. Petrasek, J. Mravec, R. Bouchard et al., “PIN proteins perform a rate-limiting function in cellular auxin efflux,” Science, vol. 312, no. 5775, pp. 914–918, 2006. View at Publisher · View at Google Scholar
  14. M. Geisler, J. J. Blakeslee, R. Bouchard et al., “Cellular efflux of auxin catalyzed by the Arabidopsis MDR/PGP transporter AtPGP1,” Plant Journal, vol. 44, no. 2, pp. 179–194, 2005. View at Publisher · View at Google Scholar
  15. C. Hunte, G. Von Jagow, and H. Schägger, Membrane Protein Purification and Crystallization: A Practical Guide, Academic Press, New York, NY, USA, 2nd edition, 2003.
  16. D. Blakey, A. Leech, G. H. Thomas, G. Coutts, K. Findlay, and M. Merrick, “Purification of the Escherichia coli ammonium transporter AmtB reveals a trimeric stoichiometry,” The Biochemical Journal, vol. 364, part 2, pp. 527–535, 2002. View at Publisher · View at Google Scholar
  17. J. L. Cereghino and J. M. Cregg, “Heterologous protein expression in the methylotrophic yeast Pichia pastoris,” FEMS Microbiology Reviews, vol. 24, no. 1, pp. 45–66, 2000. View at Publisher · View at Google Scholar
  18. S. Macauley-Patrick, M. L. Fazenda, B. McNeil, and L. M. Harvey, “Heterologous protein production using the Pichia pastoris expression system,” Yeast, vol. 22, no. 4, pp. 249–270, 2005. View at Publisher · View at Google Scholar
  19. H. Yang and A. S. Murphy, “Functional expression and characterization of Arabidopsis ABCB, AUX 1 and PIN auxin transporters in Schizosaccharomyces pombe,” Plant Journal. In press.
  20. E. R. S. Kunji, D.-J. Slotboom, and B. Poolman, “Lactococcus lactis as host for overproduction of functional membrane proteins,” Biochimica et Biophysica Acta, vol. 1610, no. 1, pp. 97–108, 2003. View at Publisher · View at Google Scholar
  21. S. Nouaille, L. A. Ribeiro, A. Miyoshi et al., “Heterologous protein production and delivery systems for Lactococcus lactis,” Genetics and Molecular Research, vol. 2, no. 1, pp. 102–111, 2003. View at Google Scholar
  22. M. Monne, K. W. Chan, D.-J. Slotboom, and E. R. S. Kunji, “Functional expression of eukaryotic membrane proteins in Lactococcus lactis,” Protein Science, vol. 14, no. 12, pp. 3048–3056, 2005. View at Publisher · View at Google Scholar
  23. K. Swarup, E. Benkova, R. Swarup et al., “The auxin influx carrier LAX3 promotes lateral root emergence,” Nature Cell Biology, vol. 10, no. 8, pp. 946–954, 2008. View at Publisher · View at Google Scholar
  24. Y. Yang, U. Z. Hammes, C. G. Taylor, D. P. Schachtman, and E. Nielsen, “High-affinity auxin transport by the AUX1 influx carrier protein,” Current Biology, vol. 16, no. 11, pp. 1123–1127, 2006. View at Publisher · View at Google Scholar
  25. E. M. Kramer, “Computer models of auxin transport: a review and commentary,” Journal of Experimental Botany, vol. 59, no. 1, pp. 45–53, 2008. View at Publisher · View at Google Scholar