Table 1: Examples of Roche (454) fusion primer sequences and a set of simple bar-coded Roche (454) fusion primer sequences based upon the and linkers in the IDT miRCat Small RNA Cloning Kit.

miRCat linker-specific PCR primers:

Forward -TGGAATTCTCGGGCACC-
Reverse -GATTGATGGTGCCTACAG -

Roche (454) fusion primers:

Forward -GCCTCCCTCGCGCCATCAGTGGAATTCTCGGGCACC-
adaptor A
Reverse -GCCTTGCCAGCCCGCTCAGGATTGATGGTGCCTACAG-

adaptor B
Simple fusion primer bar-coding scheme (6 of 256 possible sequences):

-GCCTCCCTCGCGCCATCAGGTACTGGAATTCTCGGGCACC-
-GCCTCCCTCGCGCCATCAGCGATTGGAATTCTCGGGCACC-
-GCCTCCCTCGCGCCATCAGTCGATGGAATTCTCGGGCACC-
-GCCTCCCTCGCGCCATCAGATGCTGGAATTCTCGGGCACC-
-GCCTCCCTCGCGCCATCAGCCTCTGGAATTCTCGGGCACC-
-GCCTCCCTCGCGCCATCAGTGGATGGAATTCTCGGGCACC-