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International Journal of Plant Genomics
Volume 2011 (2011), Article ID 314829, 13 pages
Review Article

Mutagenesis as a Tool in Plant Genetics, Functional Genomics, and Breeding

1Department of Plant and Environmental Sciences, Göteborg University, 40530 Göteborg, Sweden
2CropTailorAB, Erik Dahlbergsgatan 11A, 41126 Göteborg, Sweden
3Department of Chemical and Biological Engineering, Chalmers University of Technology, 41296 Göteborg, Sweden

Received 30 August 2011; Revised 2 December 2011; Accepted 15 December 2011

Academic Editor: Manuel Talon

Copyright © 2011 Per Sikora et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Plant mutagenesis is rapidly coming of age in the aftermath of recent developments in high-resolution molecular and biochemical techniques. By combining the high variation of mutagenised populations with novel screening methods, traits that are almost impossible to identify by conventional breeding are now being developed and characterised at the molecular level. This paper provides a comprehensive overview of the various techniques and workflows available to researchers today in the field of molecular breeding, and how these tools complement the ones already used in traditional breeding. Both genetic (Targeting Induced Local Lesions in Genomes; TILLING) and phenotypic screens are evaluated. Finally, different ways of bridging the gap between genotype and phenotype are discussed.