Mutagenesis as a Tool in Plant Genetics, Functional Genomics, and Breeding

Table 1

Published mutant populations in various plant species.

Species^a	Year^b	Mutagen^c	Mutation rate^d	Screening method^e	Source^f
Arabidopsis	2000	EMS	1/153 Kb	dHPLC, Li-Cor	McCallum et al. [24],; Colbert et al. [25]
Rice	2001	DEB, GR, FN	1/40 Kb (deletion)	Phenotypic (stress)	Leung et al. [16]
Lotus japonicus	2003	EMS	1/502 Kb	Li-Cor, CE	Perry et al. [26, 27]
Arabidopsis	2003	EMS	1/208 Kb	Li-Cor	Till et al. [28]
Barley	2004	EMS	1/1 Mb	dHPLC	Caldwell et al. [23]
Maize	2004	EMS	1/485 Kb	Li-Cor	Till et al. [19]
Durum wheat	2005	EMS	1/40 Kb	Li-Cor	Slade et al. [29]
Bread wheat	2005	EMS	1/24 Kb	Li-Cor	Slade et al. [29]
Rice	2005	EMS	1/1 Mb	Li-Cor	Wu et al. [17]
Rice	2007	EMS	1/294 Kb	Li-Cor	Till et al. [18]
Rice	2007	Az-MNU	1/265 Kb	Li-Cor	Till et al. [18]
Pea	2007	EMS	1/669 Kb	Li-Cor	Triques et al. [30]
Soybean	2008	EMS	1/140 Kb	Li-Cor	Cooper et al. [31]
Soybean	2008	NMU	1/140 Kb	Li-Cor	Cooper et al. [31]
Rice	2008	MNU	1/135 Kb	CE	Suzuki et al. [32]
Barley	2008	Az	1/374 Kb	Li-Cor	Talamè et al. [33]
Rapeseed	2008	EMS	1/41 Kb	Li-Cor	Wang et al. [34]
Sorghum	2008	EMS	1/526 Kb	Li-Cor	Xin et al. [35]
Bread wheat	2008	EMS	1/23 Kb	AGE	Dong et al. [36]
Tomato	2009	EMS	1/735 Kb	CE, HRM	Gady et al. [37]
Barley	2009	EMS	1/500 Kb	Li-Cor	Gottwald et al. [38]
Cabbage	2009	EMS	1/447 Kb	Li-Cor	Himelblau [39]
Medicago	2009	EMS	1/424 Kb	CE	Le Signor et al. [40]
Medicago	2009	EMS	1/485 Kb	Li-Cor	Le Signor et al. [40]
Arabidopsis	2009	EMS	1/51 Kb	Li-Cor	Martín et al. [41]
Bread wheat	2009	EMS	1/40 Kb	PAGE	Uauy et al. [42]
Bread wheat	2009	EMS	1/41 Kb	Li-Cor	Uauy et al. [42]
Arabidopsis	2010	EMS	1/415 Kb	HRM	Bush and Krysan [43]
Melon	2010	EMS	1/573 Kb	Li-Cor	Dahmani-Mardas et al. [44]
Pea	2008	EMS	1/200 Kb	Li-Cor	Dalmais et al. [45]
Oat	2010	EMS	1/30 Kb	MALDI-TOF	Chawade et al. [22]
Tomato	2010	EMS	1/322 Kb	Li-Cor	Minoia et al. [46]
Bread wheat	2010	EMS	NA	Li-Cor	Sestili et al. [47]
Brassica rapa	2010	EMS	1/44 Kb	CE	Stephenson et al. [48]
Peanut	2011	EMS	1/931 Kb	Li-Cor	Knoll et al. [49]
Peanut	2011	DES	None detected	Li-Cor	Knoll et al. [49]
Sunflower	2011	EMS	1/475 Kb	Li-Cor	Sabetta et al. [50]

^aPlant species used for developing the mutant population.
^bYear of publication.
^cMutagen used for inducing mutations—EMS (ethyl methanesulfonate), Az-MNU (sodium azide plus methylnitrosourea), Az (sodium azide), DES (diethyl sulfate), GR (Gamma Ray Bombardment), FN (Fast Neutron Bombardment), and DEB (Diepoxybutane).
^dMutation frequency.
^eMutation screening method—CE (capillary electrophoresis), HRM (high-resolution melting), AGE (agarose gel electrophoresis), and PAGE (polyacrylamide gel electrophoresis).
^fReferences.