Research Article

Poor Homologous Synapsis 1 Interacts with Chromatin but Does Not Colocalise with ASYnapsis 1 during Early Meiosis in Bread Wheat

Figure 5

TaPHS1 localisation during early meiosis in wild type bread wheat. (a) Telomere bouquet stage, (b) leptotene, (c) early zygotene, (d) zygotene, (e) late zygotene/pachytene transition, (f) pachytene, (g) diplotene. TaPHS1 (green; left panel) localises to 4′-6-diamidino-2-phenylindole- (DAPI-) stained chromatin (blue) as diffuse tracts and/or punctated foci (as seen by viewing the merged + DAPI image). Middle panels show the TaASY1 signal (red), while the panels on the right show merged TaPHS1, TaASY1, and DAPI. Arrowheads (white) represent the nucleolus. Scale bars: 7.5 μm.
514398.fig.005