Application of Phosphoproteomics to Find Targets of Casein Kinase 1 in the Flagellum of Chlamydomonas

Table 1

Phosphoproteins identified in 137c [11] whose phosphopeptides or phosphorylation sites are missing in CKI-7-treated cells.

Flagellar central pair-associated protein; PF6
Hydin-like protein; HYD3
Inner dynein arm I1 intermediate chain; IC138
Intraflagellar transport protein IFT43
Outer dynein arm docking complex subunit 1^a,b; ODA-DC1, ODA3
Radial spoke protein 17; RSP17
FAP59^c; RecF/RecN/SMC N-terminal domain
FAP116^a,d,e; microtubule-binding protein MIP-T3 domain
FAP190^a,f; sterile alpha motif
FAP228; callose synthase-like protein; 1,3-beta-glucan synthase component
FAP230; ankyrin repeats; ion transport protein domain
FAP254; putative ankyrin-like protein
FAP288; EF hand
FAP1^a,g
FAP93
FAP147
FAP184
FAP263

The function of depicted proteins is given as determined by NCBI BLASTp, along with their conserved domains.
^aNot all previously identified peptides (listed in Table S1 in [11]) are present in the CKI-7-treated cells.
^bVariants of peptide TISGADTPEEVLAYWEGLK with the phosphorylation sites Thr-345, Ser-347, and Thr-351 as well as variants of peptide ILGYTGSDVEEEEPESEEETEEEANKDDGVVDR with the phosphorylation sites Tyr-697 and Ser-709 are missing.
^cPredicted functional domains are present only in the Vs3 model.
^dVs2 model differs significantly from Vs3 model.
^eThe phosphorylation site Ser-255 in peptide SASPGGEDPLNKSGSAAPK is missing.
^fVariants of peptide STSSIGGGYSEPVGSDGEGSDAASAKPR with phosphorylation sites on Ser-370, Ser-375 and Ser-379 are missing.
^gThe phosphorylation site Ser-55 in peptide SRGSFQEGQAMVR is missing.