Phylogenetic, Molecular, and Biochemical Characterization of Caffeic Acid o-Methyltransferase Gene Family in Brachypodium distachyon

Figure 3

Comparison of amino acid sequences of four BdCOMTs (BdCOMT1, BdCOMT2, BdCOMT3, and BdCOMT4) with COMTs from Oryza sativa (OsCOMT), Zea mays (ZmCOMT), Arabidopsis thaliana (AtCOMT), and Medicago sativa (MsCOMT). Inferred from MsCOMT crystallographic structure, substrate binding residues are indicated by arrows, methyl donor binding sites by triangles, and catalytic residues by stars. An asterisk “*” indicates identical residues in all sequences. A semicolon “:” indicates strongly conserved residues (score >0.5), and a dot “.” indicates weaker conserved residues (score <0.5) [9].