Application of Microsatellite Loci for Molecular Identification of Elite Genotypes, Analysis of Clonality, and Genetic Diversity in Aspen Populus tremula L. (Salicaceae)

Table 3

Results of testing of microsatellite loci in aspen.

Locus	PCR amplification	Fragment size range (bp)	Number of alleles	Status^1	Included in testing system
ORPM14	Yes	146–162	4	P	No
ORPM18	No	—	—	N	No
ORPM36	Yes	217	1	M	No
ORPM60	No	—	—	N	No
ORPM79	No	—	—	N	No
ORPM81	No	—	—	N	No
ORPM84	No	—	—	N	No
ORPM86	Yes	204–216	4	P	No
ORPM107	No	—	—	N	No
ORPM117	Yes	218	1	M	No
ORPM158	Yes	200	1	M	No
ORPM193	Yes	182–207	6	P	Yes
ORPM202	Yes	184–193	5	P	Yes
ORPM206	Yes	190–196	3	P	Yes
ORPM220	Yes	178–198	5	P	Yes
ORPM296	Yes	201–183	4	P	Yes
ORPM312	Yes	189–201	4	P	No
ORPM366	No	—	—	N	No
ORPM371	Yes	192–200	3	P	No
ORPM372	No	—	—	N	No
ORPM415	Yes	~280	1	M	No
ORPM484	Yes	190–206	3	P	No
ORPM488	Yes	197–203	2	P	No
ORPM496	No	—	—	N	No
WPMS14	Yes	224–243	3	P	Yes
WPMS15	Yes	189–207	5	P	Yes
WPMS16	Yes	139–184	9	P	Yes
WPMS17	Yes	122–146	7	P	Yes
WPMS18	Yes	219–248	7	P	Yes
WPMS19	Yes	210–252	9	P	Yes
WPMS20	Yes	210–222	4	P	Yes
WPMS21	Yes	196–240	5	P	Yes
WPMS22	Yes	115–135	3	P	Yes

 ¹P: polymorphic, M: monomorphic, and N: no PCR amplification.