Expression of Heat Shock and Other Stress Response Proteins in Ticks and Cultured Tick Cells in Response to Anaplasma spp. Infection and Heat Shock
Figure 4
Heat shock response in I. scapularis ISE6 tick cells. ISE6 cells were incubated for 24 h at 4, 31 (normal growth conditions), and 37 prior to RNA extraction. Total RNA was extracted from two independent cultures for each condition, and HSP20 and HSP70 mRNA levels were analyzed by real-time RT-PCR. The mRNA levels were normalized against tick 16S rRNA and compared between control cells grown at 31 and cells cultured at 4 and 37 using the Student’s -test ().
