Assigning Significance in Label-Free Quantitative Proteomics to Include Single-Peptide-Hit Proteins with Low Replicates

Figure 3

Schematic representation of the two-step normalization process. The gray boxes represent the fractions in the samples. Here, samples include the replicate LC/MS runs of a sample (see Figure 1 and Table 1 for the definitions). The shades indicate the hypothetical total peptide/protein intensities in the fractions. The degrees of darkness are for illustrative purpose and do not represent the actual experimental data.