Proteomic Characterization of Cerebrospinal Fluid from Ataxia-Telangiectasia (A-T) Patients Using a LC/MS-Based Label-Free Protein Quantification Technology

Table 4

MRM transitions for biomarker candidates.

Protein name	Swiss-Prot accession number	Peptides used for MRM validation assay	MRM transitions	Observed fold-change
			1063.7 (M + 2H^+) → 634.4 (y6)
Proenkephalin-A	P01210	ELLQLSKPELPQDGTSTLR	1063.7 (M + 2H^+) → 974.5 (y9)	2.57
			1063.7 (M + 2H^+) → 1313.7 (y12)
Isoform 1 of Extracellular matrix protein 1 (ECM)			790.9 (M + 3H^+) → 588.3 (y10)
	Q16610	QHVVYGPWNLPQSSYSHLTR	790.9 (M + 3H^+) → 871.9 (y15)	1.19
			790.9 (M + 3H^+) → 953.5 (y16)
			610.1 (M + 2H^+) → 488.7 (y8^2+)
Secretogranin 2	P13521	IESQTQEEVR	610.1 (M + 2H^+) → 976.5 (y8)	1.54
		VLEYLNQEK	568.6 (M + 2H^+) → 794.4 (y6)
			568.6 (M + 2H^+) → 923.4 (y7)
			478.9 (M + 3H^+) → 560.8 (y10^2+)
		ITVVDALHEIPVK	478.9 (M + 3H^+) → 610.4 (y11^2+)	−3.38
			478.9 (M + 3H^+) → 660.9 (y12^2+)
Insulin-like growth factor binding protein 7 (IGFBP7)	Q16270	AGAAAGGPGVSGVCVCK	746.9 (M + 2H^+) → 783.4 (y7)
			746.9 (M + 2H^+) → 1036.5 (y10)
			746.9 (M + 2H^+) → 1150.6 (y12)
Isoform 1 of CD166 antigen	Q13740	SSPSFSSLHYQDAGNYVCETA	Failed to detect this peptide	N/A