The Application of a Three-Step Proteome Analysis for Identification of New Biomarkers of Pancreatic Cancer

Table 1

(a) Clinical features of pancreatic cancer patients.

No	Gender	Age (years)	UICC-stage	Tumor size (mm)	TP (g/dL)	ALB (g/dL)	Che (U/L)	T-Cho (mg/dL)
1	M	66	III	35	6.7	4.3	270	220
2	M	66	III	39	6.6	4.4	359	198
3	M	79	IV	10	6.2	3.8	179	155
4	M	71	III	18	6.8	4.0	207	210
5	M	65	III	35	7.0	3.7	189	131
6	M	78	III	50	6.6	4.4	359	198
7	M	66	IIA	30	6.3	4.1	267	176
8	M	62	IV	10	5.9	3.6	171	169
9	M	38	IA	10	6.7	4.0	289	179
10	M	50	IB	30	7.3	4.6	408	203
11	M	63	IIA	18	6.6	3.9	260	138
12	M	62	IIA	38	6.0	3.4	124	149
13	M	54	IIA	24	7.0	4.5	198	162
14	M	73	IIA	25	6.7	4.1	242	176
15	F	76	IIA	26	5.0	3.3	162	95
16	M	63	IIB	15	7.1	4.2	175	163
17	M	65	IIB	32	5.8	3.5	227	113
18	M	68	IIB	80	6.8	4.0	316	217
19	M	71	IIB	24	7.5	4.1	221	144
20	M	74	IIB	27	7.0	4.3	319	178
21	F	68	IIB	27	6.5	4.0	223	177
22	M	63	IIB	26	6.9	4.3	262	219
23	F	68	IIB	27	6.1	3.5	339	163
24	M	61	IIB	30	6.2	4.0	200	176
25	F	74	IIB	40	6.6	3.8	221	170
26	M	62	IIB	60	8.3	3.5	140	155
27	F	73	IIB	35	5.9	3.2	130	169
28	F	59	IIB	18	7.2	4.4	356	130
29	M	73	IIB	50	5.7	3.3	127	135
30	F	62	IIB	25	6.8	4.1	255	293
31	F	71	III	25	6.8	4.2	294	159
32	F	78	III	50	5.9	3.3	197	182
Ave ± SD		66±8.6		30.9±15.0	6.6±0.6	3.9±0.4	240.2±75.6	171.9±6.6

UICC: international union against cancer, M: male, F: female. TP: total protein. ALB: albumin. Che: cholinesterase. T-Cho: total cholesterol. Ave: average. SD: standard deviation.
From number 1 to 4 were used for 2-DE, from number 1 to 8 were for first western blot, from number 9 to 32 were for second western blot.

(b) Clinical features of healthy controls.

No	Gender	Age (years)	TP (g/dL)	ALB (g/dL)	Che (U/L)	T-Cho (mg/dL)
1	M	62	7.5	4.7	401	203
2	M	61	7.7	5.1	396	269
3	M	64	7.2	4.4	284	229
4	M	73	7.6	4.7	216	203
5	M	57	8.3	5.0	430	301
6	M	57	6.7	4.8	328	253
7	M	65	7.2	4.8	375	230
8	M	64	7.2	4.6	327	296
9	M	55	6.9	4.6	300	211
10	M	71	7.1	4.7	290	267
11	F	64	8.1	5.4	293	192
12	M	71	7.2	4.5	233	213
13	M	55	7.2	4.5	365	227
14	M	68	7.4	4.4	300	255
15	F	67	7.0	4.5	279	172
16	F	71	7.0	4.5	297	220
17	M	60	6.7	4.2	284	183
18	M	61	7.0	4.7	260	198
19	M	55	6.4	3.9	304	176
20	F	70	7.6	4.7	398	225
21	M	70	7.3	4.6	257	250
22	F	67	7.4	4.5	304	194
23	M	60	7.0	4.4	338	234
24	F	77	8.1	5.0	416	274
25	F	62	7.5	4.6	284	169
26	F	64	7.2	4.5	280	208
27	M	65	7.4	4.6	264	231
28	M	61	7.8	4.3	319	239
29	M	61	7.3	4.6	309	271
30	M	66	7.5	5.0	375	278
31	F	65	7.6	4.5	339	288
32	M	73	7.1	4.4	306	211
Ave ± SD		64.4±5.7	7.3±0.4	4.6±0.3	317±53.4	230.3±36.9

M: male, F: female. TP: total protein. ALB: albumin. Che: cholinesterase. T-Cho: total cholesterol. Ave: average. SD: standard deviation.
From number 1 to 4 were used for 2-0 E, from number 1 to 8 were for first western blot, from number 9 to 32 were for second western blot.