A Comprehensive Subcellular Proteomic Survey of <i>Salmonella</i> Grown under Phagosome-Mimicking versus Standard Laboratory Conditions

<table>Experimental workflow. A fractionation scheme based on differential centrifugation and Sarkosyl solubilization of membranes was combined with spheroplasting to obtain PERI, CYT, OM, and IM samples from <svg height="11.75" id="M4" style="vertical-align:-0.23206pt" version="1.1" viewbox="0 0 8.2875004 11.75" width="8.2875004" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink">
<g transform="matrix(.017,-0,0,-.017,.062,11.4)"><path d="M457 488l-30 -3q-17 148 -131 148q-53 0 -84.5 -34.5t-31.5 -82.5q0 -42 25.5 -72t74.5 -62l33 -22q63 -42 95 -85t32 -102q0 -84 -67 -137t-163 -53q-58 0 -113 22t-70 43l-4 152l27 4q4 -32 15 -62.5t31 -59.5t53.5 -47t76.5 -18q56 0 92 35t36 96q0 39 -25 70t-78 68
l-31 22q-32 23 -53.5 41.5t-45 57t-23.5 77.5q0 82 58 132.5t156 50.5q46 0 101 -17l18.5 -6t17 -6t8.5 -3q-4 -55 0 -147z" id="x1D446"></path></g>
</svg><i>. </i>Typhimurium strain 14028. Subcellular fractions were further processed prior to high-resolution LC-MS/MS analysis.</table>

International Journal of Proteomics

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Figure 1

Figure 1: A Comprehensive Subcellular Proteomic Survey of <i>Salmonella</i> Grown under Phagosome-Mimicking versus Standard Laboratory Conditions 

International Journal of Proteomics

A Comprehensive Subcellular Proteomic Survey of Salmonella Grown under Phagosome-Mimicking versus Standard Laboratory Conditions

Figure 1