Rapid Screening of the Epidermal Growth Factor Receptor Phosphosignaling Pathway via Microplate-Based Dot Blot Assays

Figure 1

The EGFR signaling cascade. The binding of EGF to the EGF receptor (EGFR) results in receptor dimerization and conformational changes triggering autophosphorylation. Under proper conditions, phosphorylated EGFR activates any number of three downstream signaling pathways through Ras, PI3K, and JAK, respectively. The work performed in this study focuses on the Ras cascade (highlighted in green). The three inhibitors (blue) are shown acting at their specific sites of signal interruption.