International Journal of Proteomics / 2012 / Article / Fig 2

Research Article

Functional Proteomic Profiling of Phosphodiesterases Using SeraFILE Separations Platform

Figure 2

Relationship between change in cAMP hydrolysis activity and protein content of the unfractionated brain homogenates and SeraFILE-generated subproteomes. The figure shows change in cAMP hydrolysis of rat brain homogenate (RBH) and generated subproteomes, (a), and bovine brain homogenate (BBH) and generated subproteomes, (b). X-axis represents protein concentration. Y-axis represents percentage change in cAMP hydrolysis activity due to the challenge of 50 μM cGMP, as compared to basal cAMP hydrolysis activity. A2, B2, D2, M2, and N2 represent subproteomes from the homogenates.

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