International Journal of Proteomics / 2012 / Article / Tab 3

Research Article

Optimization of an Efficient Protein Extraction Protocol Compatible with Two-Dimensional Electrophoresis and Mass Spectrometry from Recalcitrant Phenolic Rich Roots of Chickpea (Cicer arietinum L.)

Table 3

Proteins identified by MALDI-TOF MS analyses.

S no.Spot ID.Protein identityPeptides matchedSequence coverage (%)MOWSE scoreAccession number (NCBI)Mr(kDa)/pI
experimental
(theoretical)
Plant species

1sp 165NADP specific isocitrate dehydrogenase 1017%70Q9XGU7_ORYSA46.4/6.29
(46.0/6.0)
Oryza sativa
2sp 212Glyceraldehyde 3 phosphate dehydrogenase924%86Q6K5G8_ORYSA36.716/7.68
(37/6.5)
Oryza sativa
3sp 109Triose phosphate isomerase620%71Q38IW8_SOYBN27.4/5.87
(25/5.5)
Glycine max
4sp 55Fructokinase-like protein940%94Q8LPE5_CICAR26.26/5.03
(35.5, 4.5)
Cicer arietinum
5sp 36ATP synthase (subunit D chain)1336%88ATPQ_ARATH19.4/5.09
(20/5.0)
Arabidopsis thaliana
6sp 267Porin of Pea, channel protein211%134T1255829.7/8.56
(30/9.5)
Phaseolus coccineus
7sp 19Plasma membrane intrinsic polypeptide1038%74Q9SMK5_CICAR23.3 /4.95
(24.5/5.0)
Cicer arietinum
8sp 248Unidentified protein1135%80CAA0649122.12/9.91
(44.0,9.0)
Cicer arietinum
9sp 80Putative pyruvate dehydrogenase E1 beta subunit isoform 1 protein26%55Q6Z1G7_ORYSA40.2/5.25
(38.5/5.3)
Oryza sativa

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