Table of Contents
International Journal of Proteomics
Volume 2012 (2012), Article ID 679301, 9 pages
http://dx.doi.org/10.1155/2012/679301
Research Article

SELDI-TOF-MS Serum Profiling Reveals Predictors of Cardiac MRI Changes in Marathon Runners

1Beaumont BioBank, William Beaumont Hospital, Royal Oak, MI 48073, USA
2Department of Radiation Oncology, William Beaumont Hospital, Royal Oak, MI 48073, USA
3Department of Cardiovascular Medicine, William Beaumont Hospital, Royal Oak, MI 48073, USA

Received 24 April 2012; Accepted 29 June 2012

Academic Editor: Visith Thongboonkerd

Copyright © 2012 George D. Wilson et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Purpose. To utilize proteomics to discover proteins associated with significant cardiac magnetic resonance imaging (MRI) changes in marathon runners. Methods. Serum from 25 runners was analyzed by surface enhanced laser desorption ionization time-of-flight mass spectrometry (SELDI-TOF-MS). Proteomic profiles were compared in serum samples obtained prior to the race, at the finish line and within 7 hours after race to identify dynamic proteins correlated with cardiac MRI changes. Results. 693 protein/peptide clusters were identified using two ProteinChip surface chemistries and, of these, 116 were significantly different between the three time points. We identified 7 different patterns of protein expression change within the runners and 5 prerace protein peaks, 16 finish-line protein levels, and 15 postrace proteins which were correlated with significant postrace cardiac MRI changes. Conclusions. This study has identified baseline levels of proteins which may be predictive of risk of significant cardiac damage following a marathon race. Preliminary identification of the significant proteins suggested the involvement of cytokines and other proteins involved in stress and inflammatory response.