An Economical High-Throughput Protocol for Multidimensional Fractionation of Proteins
Figure 10
Representative examples of 2DE analysis (silver-stained) of two MDLC fractions. These are in both cases the 45% (v/v) acetonitrile reversed-phase SPE fractions of 0.25 M (a) and 0.4 M (b) potassium chloride eluted anion-exchange fractions, derived from a cytosolic DDF extract of cultured human Neuro-2A cells.