An Economical High-Throughput Protocol for Multidimensional Fractionation of Proteins

Figure 7

Exemplary linear-MALDI-TOF mass spectra of proteins in several different reversed-phase SPE fractions of a 0.25 M potassium chloride eluted anion-exchange fraction, derived from a DDF cytosolic extract of cultured human Neuro-2A cells. Proteins in fractions were directly cospotted with sinapic acid matrix and it is evident that such analysis is directly compatible with the MDLC fractionation protocol described herein.