Proteomic and Bioinformatics Analyses of Mouse Liver Microsomes

Table 5

Comparison of the current study with the literature data [18–20].

	Current study	Ref. [19]	Ref. [18]	Ref. [20]
Species	Mouse	Mouse	Mouse	Rat
Sample	Liver microsome	Liver microsome	Liver microsome	ER, Golgi
Pretreatment	None	Phenobarbital	Phenobarbital or 3-methylcholanthrene	None
Sample preparation	Subfractionated by differential ultra-centrifugation + sucrose gradient centrifugation + Na2CO3	Subfractionated by differential ultra-centrifugation	Differential ultracentrifugation	Subfractionated by differential ultra-centrifugation + density gradient centrifugation
Protein separation	2DE, 1DE	1DE	2DE	1DE
1D/2D-Gel Stain	Coomassie brilliant blue (2DE; 1DE)	—	Silver stain	—
Protein identification	MS/MS	MS/MS	PMF	MS/MS
Protein spots on 2D-Gel	514	—	1100	—
Proteins identified in 2D-Gel	183	—	139	—
Proteins identified in 1DE	99	519	—	832 (ER)
Proteins identified in 2-D LC	—	1410	—	—
Proteins identified in 3-D LC	—	3703	—	—
Total identified proteins	259	4142	Unspecified	832 (ER)
Membrane proteins	2DE: 41 (41/183 = 22%); 1DE: 54 (54/99 = 55%); Total: 62 (62/259 = 24%)	1DE: 138 (138/519 = 27%); 2-D LC: 259 (259/1410 = 21%); 3-D LC: 659 (659/3703 = 18%)	Unspecified	183 (183/832 = 22%)
Protein superfamily
P450 family members	10	29	2	11
Ribosomal proteins	13	16	Unspecified	45
UDP glycosyltransferases, UGTs	6	8	Unspecified	3
Tubulins	11	5	Unspecified	2
Short-chain  dehydrogenase/reductase	32	9	Unspecified	56
Protein disulfide isomerase	2	4	Unspecified	1