Research Article
Method for Recovery and Immunoaffinity Enrichment of Membrane Proteins Illustrated with Metastatic Ovarian Cancer Tissues
Table 1
Affinity-purified polyclonal antibodies used for western blots and their sources.
| | Biomarker | Source | Ab Catalog no. | Control cell lysate |
| | Lysophosphatidic acid receptor 2 (EDG4) | Santa Cruz Biotechnology | sc25490 | BT-20 | | Lysophosphatidic acid receptor 1 (EDG2) | Abcam | ab23698 | A549 | | CD95L, tumor necrosis factor ligand (FASLG) | Cell Signaling | 4233S | MDA-MB-231 | | CD304, neurophilin-1 (NRP1) | Santa Cruz Biotechnology | sc7329 | MDA-MB-231 | | CD309, vascular endothelial growth factor receptor 2 (KDR) | Santa Cruz Biotechnology | sc48161 | MOLT-4 | | CD63, lysosomal-associated membrane protein 3 (LAMP-3) | Santa Cruz Biotechnology | sc15363 | MOLT-4 | | Apoptosis regulator 2 (BCL2) | Cell Signaling | 2872 | MOLT-4 | | Cyclin-E2, G1/S-specific (CCNE2) | Strategic Diagnostics | 2901.00.02 | MOLT-4 | | Glypican-3 (GPC3) | Santa Cruz Biotechnology | sc11395 (30 kDa) sc10455 (60–80 kDa) | MDA-MB-231 | | RAC serine/threonine-protein kinases (pan-AKT) | R&D Systems | AF2055 | MOLT-4 | | β-tubulin (TUBB) | Santa Cruz Biotechnology | sc9935 | NIH/3T3 |
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