Research Article

Method for Recovery and Immunoaffinity Enrichment of Membrane Proteins Illustrated with Metastatic Ovarian Cancer Tissues

Table 3

The concentrations of various protein biomarkers extracted from mixed metastatic ovarian tumors obtained from the omenta of various patients during surgical debulking as measured by ELISA. Proteins were extracted from the cryogenically ground tumor samples using a Barocycler with TD2 Buffer. The concentrations (± one standard deviation) of each biomarker are determined from the extract contained using the ELISA assays described in Figure 3 and extrapolated to that present in the tumor assuming 100% recovery. Transferrin was used as a ubiquitous control protein, which is indicative of the serum content of the sample. Some biomarkers were below the detection limits of the assay. MMP3 was below the limits of quantitation of the ELISA.

Biomarker proteinExtract concentration (ng/mL)Tumor concentration (ng/g)

Holo-Tf80,000 ± 40,000
MMP2BDL (<10)BDL (<70)
MMP3*17
AKT140 (single determinant)
VEGF R2/sVEGF R20.2 (only 2 replicates)

BDL: below detection limits (assay detection limits).
*Measurements were below the quantitation limits of the assay.