Table of Contents
International Journal of Proteomics
Volume 2013, Article ID 180605, 12 pages
http://dx.doi.org/10.1155/2013/180605
Review Article

Current Status and Advances in Quantitative Proteomic Mass Spectrometry

1Bioanalytical Mass Spectrometry Facility, Mark Wainwright Analytical Centre, The University of New South Wales, Sydney, NSW 2052, Australia
2Department of Gastroenterology and Liver Services, Concord Repatriation General Hospital, Sydeny, NSW 2139, Australia

Received 14 October 2012; Revised 16 January 2013; Accepted 21 January 2013

Academic Editor: Bomie Han

Copyright © 2013 Valerie C. Wasinger et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The accurate quantitation of proteins and peptides in complex biological systems is one of the most challenging areas of proteomics. Mass spectrometry-based approaches have forged significant in-roads allowing accurate and sensitive quantitation and the ability to multiplex vastly complex samples through the application of robust bioinformatic tools. These relative and absolute quantitative measures using label-free, tags, or stable isotope labelling have their own strengths and limitations. The continuous development of these methods is vital for increasing reproducibility in the rapidly expanding application of quantitative proteomics in biomarker discovery and validation. This paper provides a critical overview of the primary mass spectrometry-based quantitative approaches and the current status of quantitative proteomics in biomedical research.