International Journal of Proteomics / 2013 / Article / Fig 5

Research Article

Top-Down Characterization of the Post-Translationally Modified Intact Periplasmic Proteome from the Bacterium Novosphingobium aromaticivorans

Figure 5

Top-down and bottom-up analysis of the hypothetical protein Saro_1314. (a) Fragmentation ion map illustrating high confidence identifications (“Q” highlighted in green font was modified as pyroglutamic acid, and two “C” highlighted in red font formed a disulfide bond). (b) Sequence coverage between top-down approach and bottom-up approach (blue arrows indicate the sequences identified using bottom-up approach).