Research Article

Proteomic Analysis and Label-Free Quantification of the Large Clostridium difficile Toxins

Table 2

Summary of the 24 most abundant C. difficile ATCC 43255 proteins identified and quantified in a commercial lyophilized culture filtrate.

ā€ƒProteingi numberMW (Da)# Unique peptides# Unique spectra# Total spectra% Sequence coverageProtein.fmolOnColumnProtein.ngramOnColumn

150S ribosomal protein L1gi 25530500424,789461528.90%6.84293782.table.002a1
250S ribosomal protein L7/L12 gi 25530500612,60210143375.20%36.5293782.table.002a2
3Acetyl-CoA acetyltransferasegi 25530598540,84323865.00%100293782.table.002a3
4Cell surface protein (S-layer precursor protein)gi 25530783179,907791978.20%376293782.table.002a4
5Electron transfer flavoprotein alpha-subunitgi 25530539837,13113173939.70%58.7293782.table.002a5
6Electron transfer flavoprotein beta-subunitgi 25530539728,6547102338.70%54.4293782.table.002a6
7Enolasegi 25530820546,07312143548.10%18.3293782.table.002a7
8Gamma-aminobutyrate metabolism dehydratase/isomerasegi 25530736455,084671217.70%10.8293782.table.002a8
9Glycine cleavage system protein Hgi 25530573214,02923618.40%15.7293782.table.002a9
10Isocaprenoyl-CoA: 2-hydroxyisocaproate CoA-transferasegi 25530539244,21833635.30%13.9293782.table.002a10
11Molecular chaperone DnaK gi 25530749166,45217235036.60%22.3293782.table.002a11
12NAD-specific glutamate dehydrogenasegi 25530517645,99844941.80%88.5293782.table.002a12
13Oligopeptide ABC transporter, substrate-binding lipoproteingi 25530585858,5132245.56%9.08293782.table.002a13
14Oligopeptide ABC transporter, substrate-binding proteingi 25530770758,12233411.80%6.84293782.table.002a14
15Putative amino acid aminotransferasegi 25530870244,78113142947.00%29.4293782.table.002a15
16Putative aminoacyl-histidine dipeptidasegi 25530570752,53322418.60%15.2293782.table.002a16
17Putative anaerobic nitric oxide reductase flavorubredoxingi 25530606044,820451124.20%5.66293782.table.002a17
18Putative propanediol utilization proteingi 25530771920,01633520.60%17.5293782.table.002a18
19Putative rubrerythringi 25530644119,99023929.30%30.7293782.table.002a19
20Putative translation inhibitor endoribonucleasegi 25530754313,582451475.40%26.2293782.table.002a20
21Subunit of O2-sensit 2-hydroxyisocaproyl-CoA dehydratasegi 25530539542,34914162633.90%21.9293782.table.002a21
22Subunit of O2-sensit 2-hydroxyisocaproyl-CoA dehydratasegi 25530539446,34522523.50%18.8293782.table.002a22
23Toxin Agi 255305655308,129556513529.90%41.3293782.table.002a23
24Toxin Bgi 255305652269,70741509628.20%26.9293782.table.002a24

293782.table.002

Proteins were identified using PLGS and Mascot search parameters and validated as greater than 95% peptide probability, min 2 peptides, 99% protein probability using Scaffold; FDR was zero. The colors in protein nanogram OnColumn are based on the estimated protein amounts.