Table of Contents
International Journal of Proteomics
Volume 2013 (2013), Article ID 581862, 16 pages
Research Article

iTRAQ-Based and Label-Free Proteomics Approaches for Studies of Human Adenovirus Infections

1Institute of Molecular Life Sciences, University of Zurich, Winterthurerstrasse 190, 8057 Zurich, Switzerland
2Life Science Zurich Graduate School, Molecular Life Science Program, Switzerland
3Functional Genomics Center Zurich, University of Zurich, Winterthurerstrasse 190, 8057 Zurich, Switzerland

Received 11 September 2012; Revised 19 December 2012; Accepted 11 January 2013

Academic Editor: Valerie Wasinger

Copyright © 2013 Hung V. Trinh et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

Supplementary Figure 1: Comparison of PF2 and PF3 for label-free quantitations. Samples were obtained from HAdV-B3-infected cells (red), and HAdV-C5-infected cells (black).

Supplementary Table 1: Pairwise comparisons for commonly quantified proteins given by log2 ratios. The ratios for the same proteins in HAdV-B3/C5-infected cells vs Ctrl were compared by different quantitation software including PL vs PP (581 proteins), PL vs Sc+ (540 proteins), PF2 vs PL (455 proteins), PF2 vs PF3 (363 proteins), PF2 vs PP (442 proteins), and PF2 vs Sc+ (1,673 proteins). (*) indicates the value derived from the quantitation software which is used for comparison. For example, PL vs PP*, (*) indicates the values of quantitation obtained from the PP quantitation software.

Supplementary Table 2: List of significantly up- or down-regulated viral and cellular proteins quantified by iTRAQ and by label-free methods. Proteins with significant (Sig) fold changes higher than 0.6 log2 ratio are shown in bold. Viral proteins encoded by HAdV-B3 and HAdV-C5 appear in red and blue, respectively; cellular proteins appear in black.

  1. Supplementary Figure 1
  2. Supplementary Table 1
  3. Supplementary Table 2