Issues and Applications in Label-Free Quantitative Mass Spectrometry
Figure 1
A typical LC-MS/MS analysis of a peptide. (a) The peptide is eluted from an LC column and its ion intensity is recorded at different time points, forming a peptide peak. The scan time points for (b) and (c) are labeled in red. (b) At scan #4036, a full MS scan is performed, and all the peptide ions including ion m/z 786.09 are recorded. (c) At scan #4037, an MS/MS scan is performed, and the ion m/z 786.09 is chosen as a precursor ion to generate product ions, providing peptide fragmentation information for peptide identification.
