A Novel Peptide-Based SILAC Method to Identify the Posttranslational Modifications Provides Evidence for Unconventional Ubiquitination in the ER-Associated Degradation Pathway

Table 1

The normalized light to heavy ratios, denoting the relative abundance of selected peptides, between high molecular weight regions of the gel (HMW: containing light ubiquitin-modified KR-TCRα) and low-molecular weight regions of the gel (LMW: containing heavy unmodified KR-TCRα). These results are representative of two independent experiments.

KR-TCRa peptides	log2 (HMW/LMW)
R.GDQVEQSPSALSLHEGTGSALR.C	1.009
R.AVQWFQQNSR.G	0.388
R.LTFGQGTVLSVIPDIQNPEPAVYQLR.D	0.242
R.ESYSTLHIR.D	0.087
R.TVLDM^∧R.A	0.074
R.TM^∧ESGTFITDR.T	0.008
R.SFETDM^∧NLNFQNLSVM^∧GLR.I	−0.536
R.LWSSYPYDVPDYAL.-	−0.618
R.VAGFNLLM^∧TLR.L	−0.780

^∧Indicates oxidized methionine.