Research Article

A Colorimetric Method for Monitoring Tryptic Digestion Prior to Shotgun Proteomics

Table 2

Percent of peptides with 1+, 2+, and 3+ charge states in 8 h and 24 h digested plasma and serum samples.

Charge state% of charged peptides in plasma% of charged peptides in serum
8 h24 h8 h24 h

1+0.931.990.763.2
2+72.4368.1685.5082.4
3+26.6429.8513.7414.4