Combined Phosphoproteomics and Bioinformatics Strategy in Deciphering Drug Resistant Related Pathways in Triple Negative Breast Cancer

Figure 2

Representative MS/MS spectra for 3 phosphopeptides. (a) MS/MS coverage of vimentin in MDA-MB-231 cell line. Peptide sequence of “SLYASsPGGVYATR” and phosphorylated serine was found upregulated in the resistant cell lines. (b) MS/MS coverage of epidermal growth factor receptor in MDA-MB-468 cell line. Peptide sequence of “ELVEPLtPSGEAPNQALLR” and phosphorylated threonine was found downregulated in the resistant cell lines. (c) MS/MS coverage of serine/arginine repetitive matrix protein 1 in HCC 1187 cell line. Peptide sequence of “KEKtPELPEPSVK” and phosphorylated threonine was found downregulated in the resistant cell lines.

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