Comparison of Heavy Labeled (SIL) Peptide versus SILAC Protein Internal Standards for LC-MS/MS Quantification of Hepatic Drug Transporters
Figure 2
LC-MRM chromatograms of a representative trypsin digest of liver tissue membrane sample. The chromatograms show elution patterns and selectivity for P-gp, OATP2B1, OATP1B3, and OATP1B1 peptides (Table 1), respectively (left to right). Q3-1 and Q3-2 represent two different MRM transitions of a peptide. The top two channels show analyte peptides and the bottom two channels show internal standards (IS) (a). Representative calibration curves (peak area calibrator/internal standard versus amount on-column of the calibrator) and LLOQs (femtomoles, on-column) of OATP1B1, OATP1B3, OATP2B1, or P-gp (b). Limits of detection (LODs) were 3–5-fold lower than LLOQs.
