The Effect of Alendronate on Proteome of Hepatocellular Carcinoma Cell Lines

Table 1

Forward and reverse primer sequences, product size, and annealing temperatures used in the study.

S. no.	Gene	Primer sequence	Tm	Product Size
1	𝛽-Actin	F: 5′GGACTTCGAGCAAGAGATGG	62.4°C	234 bp
		R: 3′AGCACTGTGTTGGCGTACAG
2	FDPS	F: 5′AGGGCAATGTGGATCTTGTC	60.4°C	180 bp
		R: 3′GAAAGAACTCCCCCATCTCC
3	FDFT1	F: 5′GGTCCCGCTGTTACACAACT	60.4°C	194 bp
		R: 3′AAAACTCTGCCATCCCAATG
4	CASP7	F: 5′AGTGACAGGTATGGGCGTTC	61.4°C	164 bp
		R: 3′CGGCATTTGTATGGTCCTCT
5	DNMT1	F: 5′GTGGGGGACTGTGTCTCTGT	62.4°C	204 bp
		R: 3′TGAAAGCTGCATGTCCTCAC
6	RAB11A	F: 5′CCACCACGACTGGCTAATTT	60°C	208 bp
		R: 3′ATCAAGGCACCATGGCTAAC
7	RND3	F: 5′GTGCTTGCATTTTTGGGTTT	60°C	226 bp
		R:3′ATCCCATGGGTCCTGATACA