Table of Contents
International Journal of Proteomics
Volume 2014, Article ID 572901, 6 pages
http://dx.doi.org/10.1155/2014/572901
Research Article

Characterisation of the Proteome of Leptospira interrogans Serovar Canicola as a Resource for the Identification of Common Serovar Immunogenic Proteins

1Animal Health and Veterinary Laboratories Agency, Addlestone, New Haw, Surrey KT15 3NB, UK
2UCL Institute of Child Health, 30 Guilford Street, London WC1N 1EH, UK

Received 15 February 2014; Revised 24 April 2014; Accepted 24 April 2014; Published 27 May 2014

Academic Editor: Andrew J. Link

Copyright © 2014 P. C. Humphryes et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Over 230 serovars of Leptospira interrogans have been identified; however few have been completely characterised. The aim of this study was to characterise the proteome of serovar Canicola and to compare this against the serovars of Copenhageni and Pomona. 2D-LC/MS analysis identified 1653 Leptospira proteins in serovar Canicola; 60 of these proteins were common to Copenhageni and Pomona, 16 of which are known to be immunogenic. This study provides the first reported proteome for serovar Canicola and suggests that proteomic comparison of different serovars could be used as a tool for identification of novel target molecules for vaccine development.