Table of Contents
International Journal of Proteomics
Volume 2014 (2014), Article ID 572901, 6 pages
http://dx.doi.org/10.1155/2014/572901
Research Article

Characterisation of the Proteome of Leptospira interrogans Serovar Canicola as a Resource for the Identification of Common Serovar Immunogenic Proteins

1Animal Health and Veterinary Laboratories Agency, Addlestone, New Haw, Surrey KT15 3NB, UK
2UCL Institute of Child Health, 30 Guilford Street, London WC1N 1EH, UK

Received 15 February 2014; Revised 24 April 2014; Accepted 24 April 2014; Published 27 May 2014

Academic Editor: Andrew J. Link

Copyright © 2014 P. C. Humphryes et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Linked References

  1. B. Adler, M. Lo, T. Seemann, and G. L. Murray, “Pathogenesis of leptospirosis: the influence of genomics,” Veterinary Microbiology, vol. 153, no. 1-2, pp. 73–81, 2011. View at Publisher · View at Google Scholar · View at Scopus
  2. Defra, Zoonoses Report: United Kingdom, 2010.
  3. G. Pappas and A. Cascio, “Optimal treatment of leptospirosis: queries and projections,” International Journal of Antimicrobial Agents, vol. 28, no. 6, pp. 491–496, 2006. View at Publisher · View at Google Scholar · View at Scopus
  4. Defra, Zoonoses Report: United Kingdom, 2008.
  5. P. N. Levett, “Leptospirosis,” Clinical Microbiology Reviews, vol. 14, no. 2, pp. 296–326, 2001. View at Publisher · View at Google Scholar · View at Scopus
  6. R. U. M. Palaniappan, Y.-F. Chang, F. Hassan et al., “Expression of leptospiral immunoglobulin-like protein by Leptospira interrogans and evaluation of its diagnostic potential in a kinetic ELISA,” Journal of Medical Microbiology, vol. 53, no. 10, pp. 975–984, 2004. View at Publisher · View at Google Scholar · View at Scopus
  7. M. C. Ooteman, A. R. Vago, and M. C. Koury, “Evaluation of MAT, IgM ELISA and PCR methods for the diagnosis of human leptospirosis,” Journal of Microbiological Methods, vol. 65, no. 2, pp. 247–257, 2006. View at Publisher · View at Google Scholar · View at Scopus
  8. K. Natarajaseenivasan, P. Vijayachari, S. Sharma, A. P. Sugunan, J. Selvin, and S. C. Sehgal, “Serodiagnosis of severe leptospirosis: evaluation of ELISA based on the recombinant OmpL1 or LipL41 antigens of Leptospira interrogans serovar autumnalis,” Annals of Tropical Medicine and Parasitology, vol. 102, no. 8, pp. 699–708, 2008. View at Publisher · View at Google Scholar · View at Scopus
  9. S. Trombert-Paolantoni, P. Thomas, F. Hermet, V. Clairet, N. Litou, and L. Maury, “Leptospirosis screening: performance of the Serion Elisa classic Leptospira IgM KIT,” Pathologie Biologie, vol. 58, no. 1, pp. 95–99, 2010. View at Publisher · View at Google Scholar · View at Scopus
  10. S. Silpasakorn, D. Waywa, S. Hoontrakul, C. Suttinont, K. Losuwanaluk, and Y. Suputtamongkol, “Performance of Leptospira immunoglobulin M ELISA and rapid immunoglobulin G immunochromatographic assays for the diagnosis of leptospirosis,” Journal of the Medical Association of Thailand, vol. 94, supplement 1, pp. S203–S206, 2011. View at Google Scholar · View at Scopus
  11. A. Eshghi, P. A. Cullen, L. Cowen, R. L. Zuerner, and C. E. Cameron, “Global proteome analysis of Leptospira interrogans,” Journal of Proteome Research, vol. 8, no. 10, pp. 4564–4578, 2009. View at Publisher · View at Google Scholar · View at Scopus
  12. J. Malmström, M. Beck, A. Schmidt, V. Lange, E. W. Deutsch, and R. Aebersold, “Proteome-wide cellular protein concentrations of the human pathogen Leptospira interrogans,” Nature, vol. 460, no. 7256, pp. 762–765, 2009. View at Publisher · View at Google Scholar · View at Scopus
  13. C. Ludwig, M. Claassen, A. Schmidt, and R. Aebersold, “Estimation of absolute protein quantities of unlabeled samples by selected reaction monitoring mass spectrometry,” Molecular & Cellular Proteomics, vol. 11, no. 3, Article ID M111.013987, 2012. View at Publisher · View at Google Scholar
  14. J. E. Nally, J. P. Whitelegge, S. Bassilian, D. R. Blanco, and M. A. Lovett, “Characterization of the outer membrane proteome of Leptospira interrogans expressed during acute lethal infection,” Infection and Immunity, vol. 75, no. 2, pp. 766–773, 2007. View at Publisher · View at Google Scholar · View at Scopus
  15. A. Schmidt, M. Beck, J. Malmström et al., “Absolute quantification of microbial proteomes at different states by directed mass spectrometry,” Molecular Systems Biology, vol. 7, article 510, 2011. View at Publisher · View at Google Scholar · View at Scopus
  16. M. L. Vieira, D. C. Pimenta, Z. M. de Morais, S. A. Vasconcellos, and A. L. T. O. Nascimento, “Proteome analysis of Leptospira interrogans virulent strain,” Open Microbiology Journal, vol. 3, pp. 69–74, 2009. View at Publisher · View at Google Scholar
  17. X.-J. Cao, J. Dai, H. Xu et al., “High-coverage proteome analysis reveals the first insight of protein modification systems in the pathogenic spirochete Leptospira interrogans,” Cell Research, vol. 20, no. 2, pp. 197–210, 2010. View at Publisher · View at Google Scholar · View at Scopus
  18. Y. Zhong, X. Chang, X.-J. Cao et al., “Comparative proteogenomic analysis of the Leptospira interrogans virulence-attenuated strain IPAV against the pathogenic strain 56601,” Cell Research, vol. 21, no. 8, pp. 1210–1229, 2011. View at Publisher · View at Google Scholar · View at Scopus
  19. S. S. Pol, P. K. Dhakephalkar, and R. S. Bharadwaj, “Characterization of leptospires using V3 region of 16S rDNA by denaturing gradient gel electrophoresis: a case study,” Indian Journal of Medical Microbiology, vol. 27, no. 4, pp. 354–357, 2009. View at Publisher · View at Google Scholar · View at Scopus
  20. D. A. Haake, M. K. Mazel, A. M. Mccoy et al., “Leptospiral outer membrane proteins OmpL1 and LipL41 exhibit synergistic immunoprotection,” Infection and Immunity, vol. 67, no. 12, pp. 6572–6582, 1999. View at Google Scholar · View at Scopus
  21. Y. Sakolvaree, S. Maneewatch, S. Jiemsup et al., “Proteome and immunome of pathogenic Leptospira spp. revealed by 2DE and 2DE-immunoblotting with immune serum,” Asian Pacific Journal of Allergy and Immunology, vol. 25, no. 1, pp. 53–73, 2007. View at Google Scholar · View at Scopus
  22. U. Kositanont, P. Saetun, C. Krittanai, G. Doungchawee, C. Tribuddharat, and V. Thongboonkerd, “Application of immunoproteomics to leptospirosis: towards clinical diagnostics and vaccine discovery,” PROTEOMICS—Clinical Applications, vol. 1, no. 4, pp. 400–409, 2007. View at Publisher · View at Google Scholar · View at Scopus
  23. F. K. Seixas, C. H. Fernandes, D. D. Hartwig, F. R. Conceição, J. A. G. Aleixo, and O. A. Dellagostin, “Evaluation of different ways of presenting LipL32 to the immune system with the aim of developing a recombinant vaccine against leptospirosis,” Canadian Journal of Microbiology, vol. 53, no. 4, pp. 472–479, 2007. View at Publisher · View at Google Scholar · View at Scopus
  24. N. G. Coldham and M. J. Woodward, “Characterization of the Salmonella typhimurium proteome by semi-automated two dimensional HPLC-mass spectrometry: detection of proteins implicated in multiple antibiotic resistance,” Journal of Proteome Research, vol. 3, no. 3, pp. 595–603, 2004. View at Publisher · View at Google Scholar · View at Scopus
  25. P. C. Humphryes, M. E. Weeks, A. Gielbert, G. Thomson, and N. G. Coldham, “Analysis of multiple Leptospira interrogans serovar Canicola vaccine proteomes and identification of LipL32 as a biomarker for potency,” Clinical and Vaccine Immunology, vol. 19, no. 4, pp. 587–593, 2012. View at Publisher · View at Google Scholar · View at Scopus
  26. J. C. Oliveros, “VENNY. An interactive tool for comparing lists with Venn Diagrams,” 2007, http://bioinfogp.cnb.csic.es/tools/venny/index.html.