A Method to Determine Lysine Acetylation Stoichiometries

<table>Methodology for determination of lysine acetylation stoichiometry. Proteins are digested with endoproteinase Arg-C, acetylated with 1,1′-<sup>13</sup>C<sub>2</sub>-acetic anhydride, and analyzed by liquid chromatography-tandem mass spectrometry. Upon MS/MS analysis, the fragmentation of acetyllysine generates diagnostic ions, at <svg height="14.85" id="M3" style="vertical-align:-0.17555pt" version="1.1" viewbox="0 0 29 14.85" width="29" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink">
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q56 202 87 347q7 33 -3 33q-8 0 -31.5 -17.5t-41.5 -35.5l-12 28q42 45 84 72t63 27q44 0 10 -124l-20 -75h2q92 121 193 178q36 21 64 21q62 0 28 -159l-8 -37h2q50 67 103.5 112t94.5 65q39 19 62 19q60 0 23 -156l-45 -189q-9 -39 2 -39q17 0 71 49z" id="x1D45A"></path></g><g transform="matrix(.017,-0,0,-.017,13.475,12.012)"><path d="M368 703l-264 -866h-60l265 866h59z" id="x2F"></path></g><g transform="matrix(.017,-0,0,-.017,20.478,12.012)"><path d="M475 445l-64 -69q-119 -128 -289 -280q27 6 81 -15q66 -26 102 -26q48 0 111 93l23 -19q-41 -84 -75.5 -116.5t-73.5 -32.5q-37 0 -147 53q-43 21 -63 7q-22 -15 -38 -41q-4 -7 -11 -7q-8 10 -8 26q0 38 47 83l292 273q-32 -5 -67 -1q-63 7 -105 7q-32 0 -53.5 -22
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</svg> 126.0913 (for endogenous acetylated lysine residues) and 127.0947 (for endogenously unmodified lysine residues) that can be used to identify and quantify acetylated peptides.</table>

International Journal of Proteomics

A Method to Determine Lysine Acetylation Stoichiometries

Figure 1