A Method to Determine Lysine Acetylation Stoichiometries
Figure 2
Application of the method to determine lysine stoichiometries for analyzing nuclear proteins. Detection and quantification of lysine acetylation on nuclear proteins from RAW 264.7 cells treated with sodium butyrate (NaBut). (a) An example of identification and quantification of an acetylated peptide derived from histone H4. (b) Western blot against acetylated lysine 12 of histone H4 (H4K12Ac) and total histone H4 (load control). The relative densitometry of each H4K12Ac, normalized by the loading control (histone H4), is shown below each lane.
