A Method to Determine Lysine Acetylation Stoichiometries

<table class="table-group" id="tab2"><tr><td><table class="table"><tr><td class="thead-hr" colspan="5"><hr/></td></tr><tr class="thead"><td align="left">Sample</td><td align="center">Total PSM*</td><td align="center">Lysine-containing PSM</td><td align="center">Spectra containing diagnostic ion <svg height="7.9499998" id="M17" style="vertical-align:-0.10033pt" version="1.1" viewbox="0 0 13.5375 7.9499998" width="13.5375" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink">
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q56 202 87 347q7 33 -3 33q-8 0 -31.5 -17.5t-41.5 -35.5l-12 28q42 45 84 72t63 27q44 0 10 -124l-20 -75h2q92 121 193 178q36 21 64 21q62 0 28 -159l-8 -37h2q50 67 103.5 112t94.5 65q39 19 62 19q60 0 23 -156l-45 -189q-9 -39 2 -39q17 0 71 49z" id="x1D45A"></path></g>
</svg>/<svg height="8.4250002" id="M18" style="vertical-align:-0.17555pt" version="1.1" viewbox="0 0 8.5874996 8.4250002" width="8.5874996" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink">
<g transform="matrix(.017,-0,0,-.017,.062,8.013)"><path d="M475 445l-64 -69q-119 -128 -289 -280q27 6 81 -15q66 -26 102 -26q48 0 111 93l23 -19q-41 -84 -75.5 -116.5t-73.5 -32.5q-37 0 -147 53q-43 21 -63 7q-22 -15 -38 -41q-4 -7 -11 -7q-8 10 -8 26q0 38 47 83l292 273q-32 -5 -67 -1q-63 7 -105 7q-32 0 -53.5 -22
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</svg> 127.09</td><td align="center">Percentage</td></tr><tr><td class="thead-hr" colspan="5"><hr/></td></tr><tr><td align="left">NaBut-repl.A</td><td align="center">6956</td><td align="center">4418</td><td align="center">3285</td><td align="center">74.3%</td></tr><tr><td align="left">NaBut-repl.B</td><td align="center">4635</td><td align="center">3059</td><td align="center">2459</td><td align="center">80.0%</td></tr><tr><td align="left">Control-repl.A</td><td align="center">6653</td><td align="center">4033</td><td align="center">3107</td><td align="center">77.0%</td></tr><tr><td align="left">Control-repl.B</td><td align="center">5533</td><td align="center">3559</td><td align="center">2819</td><td align="center">79.2%</td></tr><tr><td align="center" colspan="5"><hr/></td></tr><tr><td align="left">Total</td><td align="center">23777</td><td align="center">15069</td><td align="center">11670</td><td align="center">77.4%</td></tr><tr class="table-tr"><td colspan="5"><hr class="tbody-hr"/></td></tr></table></td></tr><tr class="table-fn"><td><svg height="13.75" id="M19" style="vertical-align:-0.0pt" version="1.1" viewbox="0 0 7.7375002 13.75" width="7.7375002" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink">
<g transform="matrix(.012,-0,0,-.012,.062,5.525)"><path d="M471 153q-22 -15 -61 -13q-25 31 -45.5 49.5t-56.5 41.5q4 -71 28 -134q-17 -33 -42 -46q-24 12 -42 46q24 63 28 134q-36 -23 -56.5 -41.5t-45.5 -49.5q-36 -2 -61 13q0 28 19 59q65 10 130 43q-65 33 -130 43q-19 31 -19 59q22 15 61 13q25 -31 45.5 -49.5t56.5 -41.5
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</svg>PSM-peptide to spectrum match.<br/></td></tr></table>

Presence of the diagnostic ion for lysine acetylation.

International Journal of Proteomics

tab2

Table 2

Table 2: A Method to Determine Lysine Acetylation Stoichiometries 