Table of Contents
International Journal of Proteomics
Volume 2015 (2015), Article ID 270438, 13 pages
Research Article

Quantitative Proteomics and Lipidomics Analysis of Endoplasmic Reticulum of Macrophage Infected with Mycobacterium tuberculosis

1Immunology Group, International Centre for Genetic Engineering and Biotechnology, Aruna Asaf Ali Marg, New Delhi 110067, India
2School of Life Sciences, Jaipur National University, Jaipur 302025, India
3Drug Discovery Research Centre, Translational Health Science & Technology Institute, Gurgaon 122016, India

Received 26 September 2014; Revised 21 January 2015; Accepted 23 January 2015

Academic Editor: Setsuko Komatsu

Copyright © 2015 Najmuddin Mohd Saquib et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

Supplementary materials contain four tables and two figures for additional information. Table S1 contains the list of 133 ER resident proteins that were identified using SILAC strategy employing Lys-6 and Lys-8 as the heavy labels. Table S2 shows the optimized collision energy (CE) used to generate the fragmentation of head groups of 8 phospholipids under study. Table S3 provides the quantitative estimation of 8 phospholipids from the peak area compared against the corresponding phospholipids standards peak area. Table S4 presents the information of the sequences and product size of the primers corresponding to CANX, PDIA4 and ERLN2. Figure S1 shows the schematic representation of the SILAC labelling method used for the quantitative proteomics approach and lastly figure S2 depicts the observed upregulation of the host cell CANX, PDIA4 and ERLN2 upon infection with H37Rv as against H37Ra.

  1. Supplementary Materials