Table of Contents
International Journal of Proteomics
Volume 2015 (2015), Article ID 587250, 10 pages
http://dx.doi.org/10.1155/2015/587250
Research Article

A Multicenter Trial Defining a Serum Protein Signature Associated with Pancreatic Ductal Adenocarcinoma

1Department of Immunotechnology and CREATE Health, Lund University, Medicon Village 406, 223 81 Lund, Sweden
2Genetic and Molecular Epidemiology Group, Spanish National Cancer Research Centre (CNIO), C/Melchor Fernández Almagro 3, 28029 Madrid, Spain
3Epithelial Carcinogenesis Group, Spanish National Cancer Research Centre (CNIO), C/Melchor Fernández Almagro 3, 28029 Madrid, Spain
4Department of Experimental and Health Sciences, University of Pompeu Fabra, Dr. Aiguader 88, 08003 Barcelona, Spain
5Hospital del Mar Medical Research Institute (IMIM), CIBERESP, UAB, Dr. Aiguader 88, 08003 Barcelona, Spain

Received 20 May 2015; Accepted 28 September 2015

Academic Editor: Djuro Josic

Copyright © 2015 Anna S. Gerdtsson et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Background. Pancreatic ductal adenocarcinoma (PDAC) is an aggressive disease with rapid tumor progression and poor prognosis. This study was motivated by the lack of sensitive and specific PDAC biomarkers and aimed to identify a diagnostic, serum protein signature for PDAC. Methods. To mimic a real life test situation, a multicenter trial comprising a serum sample cohort, including 338 patients with either PDAC or other pancreatic diseases (OPD) and controls with nonpancreatic conditions (NPC), was analyzed on 293-plex recombinant antibody microarrays targeting immunoregulatory and cancer-associated antigens. Results. Serum samples collected from different hospitals were analyzed and showed that (i) sampling from five different hospitals could not be identified as a preanalytical variable and (ii) a multiplexed biomarker signature could be identified, utilizing up to 10 serum markers that could discriminate PDAC from controls, with sensitivities and specificities in the 91–100% range. The first protein profiles associated with the location of the primary tumor in the pancreas could also be identified. Conclusions. The results demonstrate that robust enough serum signatures could be identified in a multicenter trial, potentially contributing to the development of a multiplexed biomarker immunoassay for improved PDAC diagnosis.