Comparative Proteomic Study Reveals the Molecular Aspects of Delayed Ocular Symptoms Induced by Sulfur Mustard

Table 1

Precipitation methods and ProteoMiner protein enrichment kit efficiency.

Precipitation method	Protein amount before precipitation (μg/μL)	Protein amount after precipitation (μg/μL)	Percentage of recovery (%)	Number of spots
Acetone	62.90 ± 3.67	36.96 ± 4.33	58.76 ± 7.68	410 ± 9
ACN	62.90 ± 3.67	44.02 ± 2.01	69.98 ± 5.18	392 ± 11
10% TCA/acetone	62.90 ± 3.67	28.21 ± 3.21	44.85 ± 5.73	438 ± 8
Ammonium sulphate	62.90 ± 3.67	46.47 ± 4.06	73.88 ± 7.66	375 ± 14
Chloroform/methanol	62.90 ± 3.67	50.18 ± 2.67	79.78 ± 6.29	357 ± 9
10% TCA/acetone	62.90 ± 3.67	28.21 ± 3.21	44.85 ± 5.73	617 ± 12^*
ProteoMiner protein enrichment kit	62.90 ± 3.67	20.49 ± 3.16	32.58 ± 5.36	576 ± 10^*

Protein concentration was determined by NanoDrop at 280 nm and a number of spots are counted from 7 cm and 17 cm ^*gels by SameSpots Progenesis version 3.3. The results are obtained from six 7 cm and ten 17 cm gels.