S-Nitrosylation Proteome Profile of Peripheral Blood Mononuclear Cells in Human Heart Failure
MARS analysis of differentially S-NO modified protein spots in HF subjects. Inputs to the model were protein spots that were differentially S-NO modified at with B-H correction in HF (42 spots, ) subjects with respect to NH controls (). We employed 10-fold cross-validation ((a) and (c)) and 80% testing/20% training ((c) and (d)) approaches to assess the fit of the model for testing dataset. Shown are the protein spots identified with high ranking (score >20) by CV (a) and 80/20 (b) approaches for creating the MARS model for classifying HF subjects from NH controls. Protein spots in panels (a) and (b) are identified as spot #-protein name, and RoR values (increase ↑, red; decrease ↓, blue) are plotted. The ROC curves show the prediction success of the CV (c) and 80/20 models (d). Blue curves: training data ((AUC/ROC: 1.00); red curve: testing data (AUC/ROC: 0.85 for CV and 0.714 for 80/20).