Optimization of Urea Based Protein Extraction from Formalin-Fixed Paraffin-Embedded Tissue for Shotgun Proteomics

Figure 1

Side-by-side boxplots of detected protein percent sequence coverage (a) and peptide amino acid length (b) between each set of extraction conditions. Protein sequence coverage at the 95% confidence level and peptide lengths were detected by analysis in ProteinPilot™ using urea denaturation as a special factor.

(a)

(b)